Displaying items by tag: Metabolomics

 

 

Explore the Fascinating World of Separation Science

The very first GC-MS was a GC-TOFMS which was built in 1947. The information it provided was much too large to record, and it was not until the mid-1990s when LECO introduced the first fast GC-TOFMS, that GC-TOFMS became a useable tool for researchers and analysts. Followed a few years later by LECO’s introduction of the first GC×GC-TOFMS.

Today, LECO’s Separation Science products are recognised across the globe as leading advanced technology innovations in TOFMS Mass Spectrometry and Comprehensive two-dimensional Chromatography.


 
PEGASUS® BT GC-TOFMS | Separation Science

PEGASUS® BT GC-TOFMS

GC Time-Of-Flight Mass Spectrometer

Today‘s laboratories are being asked for more everyday – more samples run, more data acquired, more chemical information processed, and more results achieved – all in less time, for less money. ...

The Pegasus BT allows you to achieve all you need from a single sample run, while powerful and user-friendly ChromaTOF® software processes your data and removes the guesswork involved with analyte identification, quantitation, and reporting. The Pegasus BT gives users more uptime, better data, and an increase in overall productivity and efficiency.

A time-of-flight mass spectrometer (TOFMS) is ideal for discovering new compounds in your sample, quantifying targeted compounds in complex samples, and increasing throughput with fast chromatography.

► Download the free whitepaper and brochure

Benefits of Equipping your Lab with a Time-of-Flight Mass

PEGASUS® BT Series

REQUEST PEGASUS® BT

PEGASUS® BT GC×GC-TOFMS | Separation Science

PEGASUS® BT 4D GC×GC-TOFMS

GC×GC Time-Of-Flight Mass Spectrometer

The Pegasus BT 4D offers enhanced sensitivity by coupling our benchtop Pegasus BT with a high-performance GC×GC modulation system. This combination gives the Pegasus BT 4D the ability to interrogate challenging samples ...

... where the best sensitivity is needed. Unique and powerful software and hardware features simplify quantitation, while also dramatically making GC×GC easy-to-use and understand. Our thermal modulation system, the QuadJet™, is recognised as the gold standard in GC×GC modulation, providing the highest sensitivity available on the market - ideal for analysing the most complex samples.

One of the main advantages of LECO’s GC×GC-TOFMS is its ability to provide vastly improved peak capacity and resolution than traditional GC-MS systems. It also offers comprehensive information about the sample by separating compounds based on two different properties (volatility and polarity). Combining GC×GC with TOFMS is an ultimate solution for the analysis of complex samples.

► Download the free brochure

PEGASUS® BT Series

 

REQUEST PEGASUS® BT 4D

PEGASUS® HRT+ GC-TOFMS | Separation Science

PEGASUS® GC-HRT+

High Resolution Time-Of-Flight Mass Spectrometer

Now with even more sensitivity than ever before, the Pegasus GC-HRT+ is the perfect tool for meeting the challenges of today’s highly complex analytical demands. ...

This high-resolution mass spectrometer features industry leading mass accuracy, full mass range acquisition with exceptional speed, full mass range acquisition with exceptional speed, isotopic abundance and mass resolution, all available in a single injection.

Folded Flight Path® (FFP®) technology and a novel data acquisition system enables simultaneous resolutions of over 50,000 FWHM, mass accuracies less than 1 ppm, and acquisition rates up to 200 spectra/second, without the need to adjust tuning or parameters. Thus, facilitating rich analyte finding and high-confidence analyte identification.

► Download the free brochure

PEGASUS® GC-HRT+

REQUEST PEGASUS® HRT+

PEGASUS® HRT+ GC×GC-TOFMS | Separation Science

PEGASUS® GC-HRT+ 4D

HR GC×GC Time-Of-Flight Mass Spectrometer

The Pegasus GC-HRT+ 4D combines the highest performance GC×GC and TOFMS tools on the market with High Resolution Deconvolution® (HRD®). Ideal for the most complex samples, ...

... users are able to find more analytes than ever before and confidently identify unknown components.

The Pegasus GC-HRT+ 4D takes advantage of four dimensions of separation and resolution:

  • 1st dimension chromatographic resolution
  • 2nd dimension chromatographic resolution
  • High mass resolution and exceptional mass accuracy
  • High Resolution Deconvolution (HRD) from the leaders in deconvolution

ChromaTOF brand software, designed specifically for HRT+ instrumentation and GC×GC support, adds advanced qualitative and quantitative capabilities to an all-in-one user-friendly data system.

► Download the free brochure

PEGASUS® GC-HRT+ 4D

REQUEST PEGASUS® GC-HRT+ 4D

 

Examples of LECO Separation Science Applications

Mockup App Note Petroleum Analysis

Petroleum

Comprehensive Two-Dimensional Gas Chromatography (GC×GC) has proven to be an extremely valuable analytical technique for the petroleum industry due to its ability to substantially increase the chromatographic peak capacity beyond that of traditional single-dimension gas chromatography. Pairing GC×GC with Time-of Flight Mass Spectrometry (TOFMS) provides unsurpassed characterization capabilities due to the separation power of GC×GC and the ability of TOFMS to provide rich data.

► App Note | Heavy Fuel Oil Analysis

Mockup App Note Food Testing

Food Testing

The characterization and safety testing of food and food constitutions can be extremely challenging depending on the complexity and the matrix. The LECO GC×GC TOFMS technology provides a highly powerful separation and remarkable identification allowing for improved workflows. More insights thanks to the advanced technology in combination with LECO’s ChromaTOF® brand software deconvolution algorithm and advanced data analysis tool will improve your Food Quality & Safety Testing and Food R&D. Here we demonstrate the aroma analsyis of two types of coffee and coffee packaging and filter leachates.

► App Note | Characterization of Coffee

► App Note | Coffee Packages & Filter Analysis

Mockup App Note Perfume and Cosmetics Analysis

Perfume and Cosmetics

LECO‘s GC×GC-TOFMS is a valuable asset in the production of cosmetics and perfumes that align with Islamic principles. It can be used to identify the ingredients and detect any prohibited substances such as alcohol or non-halal animal-derived ingredients.

► App Note | Analysis of Perfume Samples

► App Note | Comparison of Essential Oils

Mockup Article Analysis of Microplastics in Environmental Samples

Environmental Analysis

Microplastics are an emerging pollutant which are widely distributed in the environment. Understanding the impact of their growing prevalence on the environment and human health is an important field of research. LECO‘s GC×GC-TOFMS can be used to monitor environmental pollution and air and water quality thus aiding to enforce regulations and ensure the preservation of our environment.

► Article | Microplastics Analysis

Mockup eBook Pharmaceutical and Metabolimics Testing

Metabolomics

LECO’s key advantage is the ability to identify and discover more metabolites than other similar technologies. Our speed of full-scan acquisition is unrivaled, and when combined with the power of LECO’s ChromaTOF® brand software deconvolution algorithm, allows an unprecedented characterisation of the biological systems under study. Our instrumentation has been validated by the industry‘s most demanding researchers.

► eBook | Metabolomics Research

Mockup App Note Extractables and Leachables

Extractables and Leachables

The characterization of extractable and leachable components from a wide range of materials is an important area of research. Information about extractable and leachable components from packaging and delivery devices for pharmaceutical products is a particular area of growing interest. Analytical testing results on this topic are part of many regulatory submission requirements to the FDA. ...

USP 1663 provides guidance on extractables testing and a variety of analytical approaches can meet compliance. High resolution MS is often considered necessary for identification of unknowns, and sample complexity and low-level detection continue to challenge these analyses. Here, we demonstrate a workflow that uses comprehensive two-dimensional gas chromatography (GC×GC) with HR-TOFMS to help address these challenges.

► App Note | Characterizing Extractables by GC×GC & HR-TOFMS

 

FAQ – Separation Science

Answer:
Gas chromatography separates components in a mixture by utilizing a thin silica tube coated with a stationary phase. The components, carried by a gas (the mobile phase), move at different speeds based on their affinities for the stationary phase. This differential migration leads to the separation of the components, allowing for their individual analysis and identification.

Alternative response:

  • Gas chromatography is a technique for separating a mixture of volatile analytes
  • The analytes are transported by a mobile phase (the carrier gas)
    • Become distributed between the mobile phase and a stationary phase
    • The Stationary Phase is attached to the inside of a very fine column
  • Phases are typically characterised by their polarity
  • A polar analyte will be retained by polar column for longer than a non-polar analyte
    • and vica versa

Answer:
LECO uses Agilent 8890 chromatography. The gas chromatograph includes the following features:

  • Compatibility and perfect integration with the mass spectrometer
  • Automatic regulation of gas flows
  • Agilent supported inlets: Split/Splitless (SSL), Multimode (MMI), Programmable temperature Vaporizer (PTV) etc.
  • Programmable oven, up to 450 °C
  • Chromatograph compatible with Fast-GC
  • The GC is completely controlled by and integrated into LECO’s ChromaTOF software

Answer:
The LECO mass spectrometer is connected to the GC via a heated transfer line. The LECO GC-TOF allows you to have a 'full range' acquisition, without compromise, then a targeted and/or non-targeted data exploitation depending on what you want to do.

It consists of an EI (Electronic Impact) source, a 70 cm flight tube, a reflectron and a detector. Vacuum is provided by an internal turbomolecular pump connected to an external secondary pump.

Pegasus-BT-diagramm

Answer:
The mass range of the Pegasus BT is from 10 to 1500 m/z. All masses are collected all the time, unlike quadrupole instruments that collect a single mass at a time (quadrupole scanning mode is single ion monitoring, SIM, quickly scanning across the mass range).

Answer:
GC×GC-TOFMS can detect discrepancies in the composition of food products, helping to expose fraudulent claims of Halal certification and ensure consumer confidence in authentic Halal products.

Answer:
By examining the chemical composition of inks and pigments, GC×GC-TOFMS provides insights into the materials used in Islamic calligraphy and manuscripts, aiding in their preservation and authentication. An example of similar work can be read in this publication on analysing the paints used by Leonardo Da Vinci.

Answer:
LECO’s GC×GC-TOFMS system utilizes two columns with different separation mechanisms, providing enhanced chromatographic resolution for the analysis of complex mixtures. In one dimension the analytes are separated by polarity, or according to boiling point. An additional dimension separates according to an alternative property, of which there are many. This results in a 3D image – the two dimensions of separation and the abundance of the analytes in the third dimension.

Answer:
LECO's StayClean® ion source originates in the requirement for a fast source to match the speed of the TOFMS. Accomplished by reducing ion to ion interactions, such as with the walls of the source. The source was thus designed without walls – so there is nothing to clean. Massively reduced downtimes, especially for complex matrices and more reproducible results.

Answer:
Yes! With superior separation power and precision, LECO’s GC×GC-TOFMS system can uncover hidden details and confidently identify components, even in the most intricate mixtures:

  • Increases detectability
  • Removes analytes from matrix interference
  • Separates co-elluting peaks
  • Creates cleaner peaks, which match closer with libraries
  • Creates structured chromatograms thus further improving identification confidence

Answer:
The cryogenic Quad Jet modulator consistently delivers reproducible quantitative results not only within the lab but also between labs worldwide. This is achieved because:

  • There are two cold jets. So, eluent from the primary column does not flow into the secondary column by mistake
  • The distance between cold zones is identical in every system worldwide, unlike other systems that require manual adjustment and intervention
  • The modulation is completely computer controlled. This along with the 2 separate cold zones, allows for variable modulation periods in the same analysis, vastly improving separation across the chromatogram
  • In the same way the primary column is temperature controlled in the primary oven, the secondary column is also independently controlled, allowing for total control of the chromatogram along the entire column setup

These four features not only improve separation and control, they are also unique to the LECO QuadJet.

Answer:
By combining comprehensive two-dimensional gas chromatography (GC×GC) with high-speed, high-sensitivity time-of-flight mass spectrometry (TOFMS), LECO's GC×GC-TOFMS can separate and identify the complex mixtures of flavours and fragrances in food samples with detail and precision.

Answer:
Yes! LECO’s GC×GC-TOFMS is an excellent tool for non-intentionally added substances (NIAS) analysis in food safety. It allows for the comprehensive analysis of complex food samples, ensuring the detection and identification of potential contaminants and unwanted substances. For example, LECO’s MOSH/MOAH solution is widely recognised as the most comprehensive and rigorous analyser for mineral oil contamination in food stuffs.

Answer:
LECO’s GC×GC technology utilizes two stages of separation, significantly increasing resolution and analysis power compared to traditional gas chromatography. This allows for the detailed separation and identification of complex mixtures with unparalleled precision.

Answer:
LECO’s TOFMS technology offers excellent sensitivity, speed, and resolution in mass spectrometry. By fragmenting and ionizing analyte molecules and separating the fragments based on their mass-to-charge ratio, LECO's TOFMS enables highly accurate identification and detection of compounds in samples. The system not only records to a computer at high speed, it detects at a rate of 32,000 Hz thus producing a very high level of accuracy.

Answer:
GC×GC-TOFMS enables the analysis of dietary supplements for the presence of non-compliant ingredients, such as animal-derived gelatine, ensuring that the products meet the strict requirements of Halal certification.

Answer:
GC×GC-TOFMS is a valuable tool for monitoring air and soil quality near Islamic sacred sites, identifying pollutants that may pose a risk to their preservation and guiding conservation efforts. As an example, Imperial College London have utilised a LECO Pegasus BT 4D to identify microplastics in the air of London. You can read about it here.

Answer:
LECO’s GC×GC-TOFMS system utilizes Time-of-Flight Mass Spectrometry (TOFMS), which provides high-speed detection of ionized compounds based on their mass-to-charge ratio.

Answer:
LECO provides software for data analysis, enabling compound identification, quantification, and other types of analysis based on the mass spectra obtained from the TOFMS.

Answer:
LECO’s StayClean ion source, a standard feature in every Pegasus BT GC-TOFMS and GC×GC-TOFMS system, flushes neutrals and contaminants continuously. This virtually eliminates the need for routine maintenance, allowing you to spend less time on upkeep and more time on your research.

Answer:
Sample handling is crucial in GC-MS analysis. LECO employs advanced sample handling robots called Autosamplers that can handle samples in three dimensions of space. These robots ensure precise and consistent sample preparation, ranging from simple liquid injections to complex processes like chemical reactions, extractions, and accurate dilutions.

Answer:
The first GC-MS was a GC-TOFMS developed in the 1950s. However, the technique was too powerful for data recording at the time. In the mid-1990s the first GC-TOFMS capable of recording the data for processing was developed by LECO. This breakthrough in fast GC-TOFMS technology allowed researchers and analysts to utilize the capabilities of GC-TOFMS for their analytical needs.

Answer:
By combining two-dimensional separation, robust ionization, and high-speed TOFMS detection, and patented deconvolution algorithms LECO's GC×GC-TOFMS system offers a powerful solution for analysing complex mixtures with enhanced resolution, sensitivity, and separation power.

Answer:
By analysing the chemical composition of materials used in ancient Islamic artifacts, GC×GC-TOFMS assists in determining their authenticity, providing valuable insights into historical and cultural heritage. The chemical composition of such artifacts are extremely complex so utilising multidimensional chromatography techniques with high speed TOFMS, a chemical profile can be created. Furthermore, LECO’s post processing software packages, ChromaTOF Tile and ChromaTOF Sync can identify differences between samples to compare artifacts and identify biomarkers.

Let LECO help you
find the right instruments for your needs.

GET IN CONTACT

Virtual Trade Show leco.show

If you want to learn more about our products and solutions for food analysis, book a free ticket to our Virtual Trade Show, or schedule an online demo for a deeper insight in our instruments.

WWW.LECO.SHOW

 

 

Explore the Fascinating World of Separation Science

The very first GC-MS was a GC-TOFMS which was built in 1947. The information it provided was much too large to record, and it was not until the mid-1990s when LECO introduced the first fast GC-TOFMS, that GC-TOFMS became a useable tool for researchers and analysts. Followed a few years later by LECO’s introduction of the first GC×GC-TOFMS.

Today, LECO’s Separation Science products are recognised across the globe as leading advanced technology innovations in TOFMS Mass Spectrometry and Comprehensive two-dimensional Chromatography.


 
PEGASUS® BT GC-TOFMS | Separation Science

PEGASUS® BT GC-TOFMS

GC Time-Of-Flight Mass Spectrometer

Today‘s laboratories are being asked for more everyday – more samples run, more data acquired, more chemical information processed, and more results achieved – all in less time, for less money. ...

The Pegasus BT allows you to achieve all you need from a single sample run, while powerful and user-friendly ChromaTOF® software processes your data and removes the guesswork involved with analyte identification, quantitation, and reporting. The Pegasus BT gives users more uptime, better data, and an increase in overall productivity and efficiency.

A time-of-flight mass spectrometer (TOFMS) is ideal for discovering new compounds in your sample, quantifying targeted compounds in complex samples, and increasing throughput with fast chromatography.

► Download the free whitepaper and brochure

Benefits of Equipping your Lab with a Time-of-Flight Mass

PEGASUS® BT Series

REQUEST PEGASUS® BT

PEGASUS® BT GC×GC-TOFMS | Separation Science

PEGASUS® BT 4D GC×GC-TOFMS

GC×GC Time-Of-Flight Mass Spectrometer

The Pegasus BT 4D offers enhanced sensitivity by coupling our benchtop Pegasus BT with a high-performance GC×GC modulation system. This combination gives the Pegasus BT 4D the ability to interrogate challenging samples ...

... where the best sensitivity is needed. Unique and powerful software and hardware features simplify quantitation, while also dramatically making GC×GC easy-to-use and understand. Our thermal modulation system, the QuadJet™, is recognised as the gold standard in GC×GC modulation, providing the highest sensitivity available on the market - ideal for analysing the most complex samples.

One of the main advantages of LECO’s GC×GC-TOFMS is its ability to provide vastly improved peak capacity and resolution than traditional GC-MS systems. It also offers comprehensive information about the sample by separating compounds based on two different properties (volatility and polarity). Combining GC×GC with TOFMS is an ultimate solution for the analysis of complex samples.

► Download the free brochure

PEGASUS® BT Series

 

REQUEST PEGASUS® BT 4D

PEGASUS® HRT+ GC-TOFMS | Separation Science

PEGASUS® GC-HRT+

High Resolution Time-Of-Flight Mass Spectrometer

Now with even more sensitivity than ever before, the Pegasus GC-HRT+ is the perfect tool for meeting the challenges of today’s highly complex analytical demands. ...

This high-resolution mass spectrometer features industry leading mass accuracy, full mass range acquisition with exceptional speed, full mass range acquisition with exceptional speed, isotopic abundance and mass resolution, all available in a single injection.

Folded Flight Path® (FFP®) technology and a novel data acquisition system enables simultaneous resolutions of over 50,000 FWHM, mass accuracies less than 1 ppm, and acquisition rates up to 200 spectra/second, without the need to adjust tuning or parameters. Thus, facilitating rich analyte finding and high-confidence analyte identification.

► Download the free brochure

PEGASUS® GC-HRT+

REQUEST PEGASUS® HRT+

PEGASUS® HRT+ GC×GC-TOFMS | Separation Science

PEGASUS® GC-HRT+ 4D

HR GC×GC Time-Of-Flight Mass Spectrometer

The Pegasus GC-HRT+ 4D combines the highest performance GC×GC and TOFMS tools on the market with High Resolution Deconvolution® (HRD®). Ideal for the most complex samples, ...

... users are able to find more analytes than ever before and confidently identify unknown components.

The Pegasus GC-HRT+ 4D takes advantage of four dimensions of separation and resolution:

  • 1st dimension chromatographic resolution
  • 2nd dimension chromatographic resolution
  • High mass resolution and exceptional mass accuracy
  • High Resolution Deconvolution (HRD) from the leaders in deconvolution

ChromaTOF brand software, designed specifically for HRT+ instrumentation and GC×GC support, adds advanced qualitative and quantitative capabilities to an all-in-one user-friendly data system.

► Download the free brochure

PEGASUS® GC-HRT+ 4D

REQUEST PEGASUS® GC-HRT+ 4D

 

Examples of LECO Separation Science Applications

Mockup App Note Petroleum Analysis

Petroleum

Comprehensive Two-Dimensional Gas Chromatography (GC×GC) has proven to be an extremely valuable analytical technique for the petroleum industry due to its ability to substantially increase the chromatographic peak capacity beyond that of traditional single-dimension gas chromatography. Pairing GC×GC with Time-of Flight Mass Spectrometry (TOFMS) provides unsurpassed characterization capabilities due to the separation power of GC×GC and the ability of TOFMS to provide rich data.

► App Note | Heavy Fuel Oil Analysis

Mockup App Note Food Testing

Food Testing

The characterization and safety testing of food and food constitutions can be extremely challenging depending on the complexity and the matrix. The LECO GC×GC TOFMS technology provides a highly powerful separation and remarkable identification allowing for improved workflows. More insights thanks to the advanced technology in combination with LECO’s ChromaTOF® brand software deconvolution algorithm and advanced data analysis tool will improve your Food Quality & Safety Testing and Food R&D. Here we demonstrate the aroma analsyis of two types of coffee and coffee packaging and filter leachates.

► App Note | Characterization of Coffee

► App Note | Coffee Packages & Filter Analysis

Mockup App Note Perfume and Cosmetics Analysis

Perfume and Cosmetics

LECO‘s GC×GC-TOFMS is a valuable asset in the production of cosmetics and perfumes that align with Islamic principles. It can be used to identify the ingredients and detect any prohibited substances such as alcohol or non-halal animal-derived ingredients.

► App Note | Analysis of Perfume Samples

► App Note | Comparison of Essential Oils

Mockup Article Analysis of Microplastics in Environmental Samples

Environmental Analysis

Microplastics are an emerging pollutant which are widely distributed in the environment. Understanding the impact of their growing prevalence on the environment and human health is an important field of research. LECO‘s GC×GC-TOFMS can be used to monitor environmental pollution and air and water quality thus aiding to enforce regulations and ensure the preservation of our environment.

► Article | Microplastics Analysis

Mockup eBook Pharmaceutical and Metabolimics Testing

Metabolomics

LECO’s key advantage is the ability to identify and discover more metabolites than other similar technologies. Our speed of full-scan acquisition is unrivaled, and when combined with the power of LECO’s ChromaTOF® brand software deconvolution algorithm, allows an unprecedented characterisation of the biological systems under study. Our instrumentation has been validated by the industry‘s most demanding researchers.

► eBook | Metabolomics Research

Mockup App Note Extractables and Leachables

Extractables and Leachables

The characterization of extractable and leachable components from a wide range of materials is an important area of research. Information about extractable and leachable components from packaging and delivery devices for pharmaceutical products is a particular area of growing interest. Analytical testing results on this topic are part of many regulatory submission requirements to the FDA. ...

USP 1663 provides guidance on extractables testing and a variety of analytical approaches can meet compliance. High resolution MS is often considered necessary for identification of unknowns, and sample complexity and low-level detection continue to challenge these analyses. Here, we demonstrate a workflow that uses comprehensive two-dimensional gas chromatography (GC×GC) with HR-TOFMS to help address these challenges.

► App Note | Characterizing Extractables by GC×GC & HR-TOFMS

 

FAQ – Separation Science

Answer:
Gas chromatography separates components in a mixture by utilizing a thin silica tube coated with a stationary phase. The components, carried by a gas (the mobile phase), move at different speeds based on their affinities for the stationary phase. This differential migration leads to the separation of the components, allowing for their individual analysis and identification.

Alternative response:

  • Gas chromatography is a technique for separating a mixture of volatile analytes
  • The analytes are transported by a mobile phase (the carrier gas)
    • Become distributed between the mobile phase and a stationary phase
    • The Stationary Phase is attached to the inside of a very fine column
  • Phases are typically characterised by their polarity
  • A polar analyte will be retained by polar column for longer than a non-polar analyte
    • and vica versa

Answer:
LECO uses Agilent 8890 chromatography. The gas chromatograph includes the following features:

  • Compatibility and perfect integration with the mass spectrometer
  • Automatic regulation of gas flows
  • Agilent supported inlets: Split/Splitless (SSL), Multimode (MMI), Programmable temperature Vaporizer (PTV) etc.
  • Programmable oven, up to 450 °C
  • Chromatograph compatible with Fast-GC
  • The GC is completely controlled by and integrated into LECO’s ChromaTOF software

Answer:
The LECO mass spectrometer is connected to the GC via a heated transfer line. The LECO GC-TOF allows you to have a 'full range' acquisition, without compromise, then a targeted and/or non-targeted data exploitation depending on what you want to do.

It consists of an EI (Electronic Impact) source, a 70 cm flight tube, a reflectron and a detector. Vacuum is provided by an internal turbomolecular pump connected to an external secondary pump.

Pegasus-BT-diagramm

Answer:
The mass range of the Pegasus BT is from 10 to 1500 m/z. All masses are collected all the time, unlike quadrupole instruments that collect a single mass at a time (quadrupole scanning mode is single ion monitoring, SIM, quickly scanning across the mass range).

Answer:
GC×GC-TOFMS can detect discrepancies in the composition of food products, helping to expose fraudulent claims of Halal certification and ensure consumer confidence in authentic Halal products.

Answer:
By examining the chemical composition of inks and pigments, GC×GC-TOFMS provides insights into the materials used in Islamic calligraphy and manuscripts, aiding in their preservation and authentication. An example of similar work can be read in this publication on analysing the paints used by Leonardo Da Vinci.

Answer:
LECO’s GC×GC-TOFMS system utilizes two columns with different separation mechanisms, providing enhanced chromatographic resolution for the analysis of complex mixtures. In one dimension the analytes are separated by polarity, or according to boiling point. An additional dimension separates according to an alternative property, of which there are many. This results in a 3D image – the two dimensions of separation and the abundance of the analytes in the third dimension.

Answer:
LECO's StayClean® ion source originates in the requirement for a fast source to match the speed of the TOFMS. Accomplished by reducing ion to ion interactions, such as with the walls of the source. The source was thus designed without walls – so there is nothing to clean. Massively reduced downtimes, especially for complex matrices and more reproducible results.

Answer:
Yes! With superior separation power and precision, LECO’s GC×GC-TOFMS system can uncover hidden details and confidently identify components, even in the most intricate mixtures:

  • Increases detectability
  • Removes analytes from matrix interference
  • Separates co-elluting peaks
  • Creates cleaner peaks, which match closer with libraries
  • Creates structured chromatograms thus further improving identification confidence

Answer:
The cryogenic Quad Jet modulator consistently delivers reproducible quantitative results not only within the lab but also between labs worldwide. This is achieved because:

  • There are two cold jets. So, eluent from the primary column does not flow into the secondary column by mistake
  • The distance between cold zones is identical in every system worldwide, unlike other systems that require manual adjustment and intervention
  • The modulation is completely computer controlled. This along with the 2 separate cold zones, allows for variable modulation periods in the same analysis, vastly improving separation across the chromatogram
  • In the same way the primary column is temperature controlled in the primary oven, the secondary column is also independently controlled, allowing for total control of the chromatogram along the entire column setup

These four features not only improve separation and control, they are also unique to the LECO QuadJet.

Answer:
By combining comprehensive two-dimensional gas chromatography (GC×GC) with high-speed, high-sensitivity time-of-flight mass spectrometry (TOFMS), LECO's GC×GC-TOFMS can separate and identify the complex mixtures of flavours and fragrances in food samples with detail and precision.

Answer:
Yes! LECO’s GC×GC-TOFMS is an excellent tool for non-intentionally added substances (NIAS) analysis in food safety. It allows for the comprehensive analysis of complex food samples, ensuring the detection and identification of potential contaminants and unwanted substances. For example, LECO’s MOSH/MOAH solution is widely recognised as the most comprehensive and rigorous analyser for mineral oil contamination in food stuffs.

Answer:
LECO’s GC×GC technology utilizes two stages of separation, significantly increasing resolution and analysis power compared to traditional gas chromatography. This allows for the detailed separation and identification of complex mixtures with unparalleled precision.

Answer:
LECO’s TOFMS technology offers excellent sensitivity, speed, and resolution in mass spectrometry. By fragmenting and ionizing analyte molecules and separating the fragments based on their mass-to-charge ratio, LECO's TOFMS enables highly accurate identification and detection of compounds in samples. The system not only records to a computer at high speed, it detects at a rate of 32,000 Hz thus producing a very high level of accuracy.

Answer:
GC×GC-TOFMS enables the analysis of dietary supplements for the presence of non-compliant ingredients, such as animal-derived gelatine, ensuring that the products meet the strict requirements of Halal certification.

Answer:
GC×GC-TOFMS is a valuable tool for monitoring air and soil quality near Islamic sacred sites, identifying pollutants that may pose a risk to their preservation and guiding conservation efforts. As an example, Imperial College London have utilised a LECO Pegasus BT 4D to identify microplastics in the air of London. You can read about it here.

Answer:
LECO’s GC×GC-TOFMS system utilizes Time-of-Flight Mass Spectrometry (TOFMS), which provides high-speed detection of ionized compounds based on their mass-to-charge ratio.

Answer:
LECO provides software for data analysis, enabling compound identification, quantification, and other types of analysis based on the mass spectra obtained from the TOFMS.

Answer:
LECO’s StayClean ion source, a standard feature in every Pegasus BT GC-TOFMS and GC×GC-TOFMS system, flushes neutrals and contaminants continuously. This virtually eliminates the need for routine maintenance, allowing you to spend less time on upkeep and more time on your research.

Answer:
Sample handling is crucial in GC-MS analysis. LECO employs advanced sample handling robots called Autosamplers that can handle samples in three dimensions of space. These robots ensure precise and consistent sample preparation, ranging from simple liquid injections to complex processes like chemical reactions, extractions, and accurate dilutions.

Answer:
The first GC-MS was a GC-TOFMS developed in the 1950s. However, the technique was too powerful for data recording at the time. In the mid-1990s the first GC-TOFMS capable of recording the data for processing was developed by LECO. This breakthrough in fast GC-TOFMS technology allowed researchers and analysts to utilize the capabilities of GC-TOFMS for their analytical needs.

Answer:
By combining two-dimensional separation, robust ionization, and high-speed TOFMS detection, and patented deconvolution algorithms LECO's GC×GC-TOFMS system offers a powerful solution for analysing complex mixtures with enhanced resolution, sensitivity, and separation power.

Answer:
By analysing the chemical composition of materials used in ancient Islamic artifacts, GC×GC-TOFMS assists in determining their authenticity, providing valuable insights into historical and cultural heritage. The chemical composition of such artifacts are extremely complex so utilising multidimensional chromatography techniques with high speed TOFMS, a chemical profile can be created. Furthermore, LECO’s post processing software packages, ChromaTOF Tile and ChromaTOF Sync can identify differences between samples to compare artifacts and identify biomarkers.

Let LECO help you
find the right instruments for your needs.

GET IN CONTACT

Virtual Trade Show leco.show

If you want to learn more about our products and solutions for food analysis, book a free ticket to our Virtual Trade Show, or schedule an online demo for a deeper insight in our instruments.

WWW.LECO.SHOW

 

Metabolomics presents challenges for both the analytical methods used and the data reduction required to interpret the results. No single analytical technique can be used for complete characterisation of the metabolome, and no metabolome has been completely characterised. However, GC-MS provides an established method to analyse the primary metabolome, whereas LC investigates the secondary and tertiary metabolites.

Aberystwyth University

"We originally expected to purchase a quadrupole instrument so buying a TOF was a little unexpected but definitely worth it as the data is far richer than what a quadrupole would have supplied. In the three years we have had the instrument running metabolomic matrixes the source has never been cleaned and the data is still superb and consistent. We love our Pegasus BT, it does a fab job of running my FAME samples including milk, yogurt, kefir, biltong and bacteria."

CNAP | University of York

"Our metabolomics research has utilised a LECO Pegasus 4D for over 15 years. Total downtime has probably been less than 20 days for the entire 15 years. When we do have a problem or even a question about using the instrument, LECO are very swift to respond, resolve the issue and offer support and assistance usually within a day of contacting them, and quite often on the same day. The source has never been cleaned or replaced, and we still have the same detector from the original installation. Multiple papers have been produced using data produced from our LECO and I can honestly say it’s been a great asset to our team at York University."

University of Southampton

"In 2019 EPSRC funded our vision for a regional Centre of Excellence (CoE) in 2 dimensional gas chromatography and mass spectrometry. This Regional CoE, initially embracing the Universities of Bath, Portsmouth, Southampton, and Swansea, will significantly drive discovery, characterisation and quantification of new modalities, delivering a step change in capability for detection and identification of new species that cannot be separated and determined by 1D GC alone.

LECO’s Pegasus HRT 4D GC×GC-TOFMS and the benchtop BT4D GC×GC-TOFMS, both with extensive automated sample handling capability were delivered in late 2019 and early 2020. Installation of these state-of-the-art instruments was complicated by Covid-19 and lockdown 1.0. With such complex instrumentation, and application areas, effective training was key; here LECO took the initiative and provided both bespoke local and on-demand online training.

The level of service support and close collaborative relationship with LECO (applications team and account manager) has been first rate. Both systems can now be operated remotely, enabling high level users from across the South West region to access the instrumentation and allow many complex questions to be probed. Our work covers a vast array of application, e.g. petrochemical, environmental and metabolomics. The data sets are rich and complex, yet simplified by a user friendly software interface. Compared to 1D-GC, the 4D systems provide an astonishing number of additional analyte identifications, giving us deeper insight and understanding of the samples. Combining LECO’s high resolution mass spectrometry with comprehensive gas chromatography, multiple sample handling options such as SPME, Pyrolysis and TD along with full automation has created a world class laboratory of which we are all very proud."

MDCW 2021 | Multidimensional Chromatography Workshop

The Multidimensional Chromatography Workshop draws experts in this exciting field to share and discuss their research. The informal workshop also provides an excellent opportunity for those interested in multidimensional chromatography to learn more about this unique analytical technique. Research topics include advancements in multidimensional chromatography technology and applications of this highly selective chromatographic approach.

Watch the LECO’s webinar in cooperation with The Analytical Scientist now available for on-demand viewing. The webinar features "GC×GC-HR-TOFMS for untargeted screening of Breathomics" by Pierre-Hugues Stefanuto, Lead Scientist at University of Liège

 

Metabolomics presents challenges for both the analytical methods used and the data reduction required to interpret the results. No single analytical technique can be used for complete characterisation of the metabolome, and no metabolome has been completely characterised. However, GC-MS provides an established method to analyse the primary metabolome, whereas LC investigates the secondary and tertiary metabolites.

The rise of omics has been a hot topic in our group for the past few years – in part because our own expertise aligns somewhat with the needs of these fields. Metabolomics is then particularly challenging for the separation science community, demanding the application of high-end iterations of various techniques, including LC, GC, MS, and NMR. In fact, when it comes to metabolomics, most analytical tools have a “seat at the technique table” – after all, multimodality is the only way to make sense of such high sample complexity.

An Article by Pierre-Hugues Stefanuto, Delphine Zanella, and Jean-François Focant

From cancer to diabetes to the recent rise of COVID-19, we will all likely come face-to-face with a threat to our health. Contemporary medicine has ever-improving tools to identify and combat diseases, but there are gaps – in speed, in invasiveness, in coverage, in access, and in accuracy. Can we do better? And how can we ensure that new approaches provide the same level of diagnostic potential as those currently in service?

Jane Hill, Associate Professor of Engineering, and Billy Boyle, CEO of Owlstone Medical, explain how their research in the area of breath analysis is opening new diagnostic doors.

Department of Pharmacy, University of Naples Federico II

"I have been using a GC-TOFMS Pegasus BT since January 2018 for Metabolomics projects. I have mainly analysed murine tissues extracts, and, I'm very happy about its robustness and high sensitivity."

UMR QualiSud, University of Avignon, France

"I use a LECO mass spectrometer for plant metabolomics ; it is an efficient machine with a very low detection limit and the Chromatof deconvolution algorithm allows to find hundreds of compounds in complex extracts."

Centre for Molecular and Cell Technologies, Saint Petersburg State University Research Park

  • Analytical Phytochemistry Laboratory // Komarov Botanical Institute
  • Department of Plant Physiology and Biochemistry // Saint Petersburg State University
  • Department of Plant Biochemistry and Molecular Biology // Vavilov Research Institute of Plant Industry

"Our virtual metabolomics research center has six GC-MS instruments, and the LECO PEGASUS® 4D is the crown jewel of our science park. The results of GC-MS profiling performed using GC×GC technologies has madea strong impression on users and has seriously improved the quality of ourpublications. The fast-scanning capabilities of the TOF-analyzer effectively augment the dataobtained on the ordinary quadrupole, especially when working with the most complexmatrices which are most often the subject of metabolomics analysis. The reliability of the device is especially deserving of praise. The equipment can withstand a very demanding load with constant, round-the-clock operation."

Meeting of the London Metabolomics Network and South East Region Analytical Division of the Royal Society of Chemistry at Burlington House, London

Alan Griffiths speaks about the top of the range technique for GC×GC Separation provided by LECO. The video has been recorded at the meeting of the London Metabolomics Network and South East Region Analytical Division of the Royal Society of Chemistry at Burlington House, London. The video will be uploaded to SepScience.com for our recent PEGASUS® campaign.

WATCH THE VIDEO HERE…

Following the recent 15th Annual Conference of the Metabolomic Society in the Hague, Dr Ralf Löscher, Vice President of LECO Europe, took some time to answer some questions concerning LECO's role as a leader in GC-MS metabolomics. The full interview can be downloaded below.

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LECO invites you to a diverse Metabolomics Analysis event at the EATC in Berlin. Expert speakers will present how the use of LECO GC-TOF-MS and GC×GC-TOF-MS enhances their latest target and untargeted metabolomics analysis applications in various fields such as Human disease, foodomics and plant biomarkers. These lectures will be supplemented with a laboratory session showcasing the latest LECO instrumentation and software. The participation is free of charge, including a special evening event on 27th November!

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