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Displaying items by tag: TOFMS
I started working with the PEGASUS GC-HRT+ 4D back in my student years. As a novice researcher, with no experience working with similar devices and no training, it was not easy at first. However, after years of working with this instrument and when compared with other sophisticated equipment in the field of gas chromatography - high-resolution mass spectrometry, I can confidently say that the ChromaTOF is the most user-friendly software of all those available now. Such a variety of functions, as well as very clear, convenient, and fast operation, combined with high-tech equipment, allows to get very decent results.
Over the past 5 years of using the Pegasus GC-HRT+ 4D, we have been able to conduct many various environmental studies, identifying trace amounts of substances in the cleanest Arctic environmental objects. At the same time, such a powerful combination of two-dimensional gas chromatography with time-of-flight mass spectrometry made it possible to analyze complex matrices, namely peat combustion products, herbal and tobacco cigarette smoke, petroleum and much more.
It is worth noting the work of the support service, which helped us very quickly and efficiently. I would like to thank the engineers for their work, who were on call almost 24/7. There are still many projects and tasks ahead that will undoubtedly be solved with the help of the Pegasus GC-HRT+ 4D.
Microplastics are a ubiquitous presence in the world, found from Arctic snow to Antarctic ice and everywhere in between. There are trillions of microplastic particles floating on surface water, and everyone from infants to adults are presumed to be ingesting anywhere from dozens to tens of thousands of these particles every day.
The first Time-of-Flight (TOF) mass spectrometer was proposed in 1946, and the design has been refined and iterated upon every since.
With an unlimited mass range visible in each spectrum and a high spectral generation speed, the advantages of TOF mass spectrometry were immediately clear. Though the instruments improved, they did eventually hit physical limitations that restricted the resolving power. More work had to be done.
With these fundamentals you will learn all the ins and outs on the LC–GC coupling, GC×GC and Time-of-Flight MS to able to determine MOSH and MOAH in food products. Please register for our presentations.
The rise of omics has been a hot topic in our group for the past few years – in part because our own expertise aligns somewhat with the needs of these fields. Metabolomics is then particularly challenging for the separation science community, demanding the application of high-end iterations of various techniques, including LC, GC, MS, and NMR. In fact, when it comes to metabolomics, most analytical tools have a “seat at the technique table” – after all, multimodality is the only way to make sense of such high sample complexity.
An Article by Pierre-Hugues Stefanuto, Delphine Zanella, and Jean-François Focant
From cancer to diabetes to the recent rise of COVID-19, we will all likely come face-to-face with a threat to our health. Contemporary medicine has ever-improving tools to identify and combat diseases, but there are gaps – in speed, in invasiveness, in coverage, in access, and in accuracy. Can we do better? And how can we ensure that new approaches provide the same level of diagnostic potential as those currently in service?
Jane Hill, Associate Professor of Engineering, and Billy Boyle, CEO of Owlstone Medical, explain how their research in the area of breath analysis is opening new diagnostic doors.
Find out how the use of Comprehensive Two-Dimensional Gas Chromatography (GC×GC) with Time-of-Flight Mass Spectrometry (TOF-MS) demonstrates excellent matrix-analyte and analyte-analyte separation quality of a high number of target pesticides, at high sensitivity.
The LECO Pegasus BT 4D was used to develop a robust and straightforward quantification workflow adhering to SANTE requirements.
National Centre for AtmosphericScience (NCAS), University of York
"We send instruments into the harshest of environments and run very demanding samples on them. Bearing this in mind, our decision to upgrade the GC-MS at our remote Cape-Verde site to the new LECO PEGASUS® BT was driven by the promise of the StayClean® source. This minimizes down time and eliminates operations which are difficult to carry out when working with remote systems. The increased sensitivity of theinstrument enables us to take smaller sample volumes which again assists with easing the burden placedon the analytical machine. Finally! the upgrade from quadrupole to time of flight mass spec will enable us to keep historical datasets which can be interrogated for any compounds which prove interesting in the future."
Department of Civil Engineering University of Strathclyde
"In 2013, I became responsible for overseeing the department’s analytical suite which included a LECO PEGASUS® 4D GC×GC TOF MS as our flagshipinstrument. I had no experience in gas chromatography and found even a standard GCMS daunting. Over the past years, I received extensive support from LECO. Especially withsupport from LECO UK the instrument is still running strong a decade after purchase. I anticipate it will go for another decade and then some."
SGS Institut Fresenius GmbH Berlin, Germany
"We at SGS MOSH/MOAH group are using the GC × GC-TOFMS from LECO to separate the highly complex fractions of MOSH/MOAH contaminates in food. When you want to identify special patterns of contamination species amongst other analytics (e. g carotenoids) you need a high separation power in combination with a fast screening MS. This is provided by the LECO TOF. Actually we have a new project together with LECO and another company to quantify MOSH/MOAH fractions. The complexity of the instrumentation is high, but with the support of the application team from LECO we reach our goals."
Environment Reference Lab, APA – Agência Portuguesa do Ambiente
"Initially we had some fears about whether the option of the time of flight detector would be the best for our laboratory, since we have no other system of this kind installed. However, at the end of 6 months of operation with the GC-TOFMS system, it was widely recognized internally that we made the right choice. An easy-to-operate system with high sensitivity, reproducibility and severalanalysis options for a large universe of analytes, and in the most varied matrices increases the versatility of laboratory operation as well as the possibility of responding to the most demanding customer requests."
Centre for Molecular and Cell Technologies, Saint Petersburg State University Research Park
- Analytical Phytochemistry Laboratory // Komarov Botanical Institute
- Department of Plant Physiology and Biochemistry // Saint Petersburg State University
- Department of Plant Biochemistry and Molecular Biology // Vavilov Research Institute of Plant Industry
"Our virtual metabolomics research center has six GC-MS instruments, and the LECO PEGASUS® 4D is the crown jewel of our science park. The results of GC-MS profiling performed using GC×GC technologies has madea strong impression on users and has seriously improved the quality of ourpublications. The fast-scanning capabilities of the TOF-analyzer effectively augment the dataobtained on the ordinary quadrupole, especially when working with the most complexmatrices which are most often the subject of metabolomics analysis. The reliability of the device is especially deserving of praise. The equipment can withstand a very demanding load with constant, round-the-clock operation."
Our new eBook summarises a large part of LECO’s application work in the field of food testing. In addition to the Application Compendium, the eBook includes an introduction to GC×GC- and TOFMS-technology, followed by an overview of the different hardware and software solutions that were used for the food testing applications. A bibliography with additional references can be found in the appendix.
The eBook is downloadable at SeparationScience now.
Download the eBook here…
Ralf Löscher speaks about the latest technologies, as well as many years experience LECO has in the field of TOFMS instrumentation. The special focus of this interview refers to the food industry and the special benefits LECO offers to this market.
“In the field of chromatographic separation science technology, LECO offers GC coupled to time-of-flight (TOF) mass spectrometry (MS). This includes one-dimensional as well as two-dimensional chromatography (GC×GC), which increases chromatographic resolution and sensitivity. In addition, our latest technology is competitively priced in relation to triple quadrupole alternatives. Furthermore, GC-TOF-MS technology is basically unbeatable for the analysis of residues in complex matrices such as teas, spices, oily products, but also baby food.“
eFoodLab // Issue 02/2019 // Interview with Ralf Löscher